WORLD TOTAL . . . . . . . 88,467 27,343 944,412 309,947...
United Kingdom . . . . . 1,887 2,300 26,823 23,585
U.S. Imports for Consumption: December 1998 and 1998 Year-to-Date
Subheading 300290: HUMAN BLOOD; ANIMAL BLOOD PREPARED FOR THERAPEUTIC, ETC.
USES; TOXINS, CULTURES OF MICRO-ORGANISMS (EXCLUDING YEASTS) AND SIMILAR
PRODUCTS NESOI
<--- ---="" 1998="" country="" dec="" div="" quantity="" value="" ytd="">
=================================================================
WORLD TOTAL . . . . . . . 36,178 643 250,982 11,604...
United Kingdom . . . . . 584 39 11,292 588
snip...see more here ;
-------- Original Message --------
Subject: TSE Advisory Committee October 14-15, 2004
Date: Fri, 10 Sep 2004 16:34:24 –0500
From: "Terry S. Singeltary Sr."
To: Bovine Spongiform Encephalopathy
Transmissible Spongiform Encephalopathies Advisory Committee
Committee Roster
Suzette Priola PhD, National Institute of Allergy & Infectious Diseases
(Chair) 1/31/05 Val Bias, National Hemophilia Foundation 1/31/07 Lynn Creekmore
DVM, U.S. Department of Agriculture 1/31/07 Lisa Ferguson DVM, U.S. Department
of Agriculture Animal & Plant Health Inspection Service 1/31/04 R. Nick
Hogan MD/PhD, University of Texas Southwestern Medical School 1/31/07 Rima
Khabbaz MD, National Center for Infectious Disease Shirley Walker, Dallas Urban
League (Consumer Representative) 1/31/04 John Bailar MD/PhD, University of
Chicago 1/31/05 Arthur Bracey MD, St. Luke's Episcopal Hospital 1/31/07 Stephen
DeArmond MD/PhD, University of California San Francisco School of Medicine
1/31/05 Pierluigi Gambetti MD, Case Western Reserve University 1/31/05 Richard
Johnson MD, Johns Hopkins University Schoo of Medicine 1/31/06 Stephen Petteway
PhD, Bayer (Non-voting Industry Representative) 1/31/05 Sidney Wolfe MD, Public
Citizen's Health Research Group 1/31/04 William Freas PhD, FDA (Executive
Secretary)
Tentative Meetings
October 14-15, 2004
Upcoming Meetings Date
Plasma-Derived Product Label Claims For TSE Testing; BSE Diagnosis, Food
Rules; vCJD Transmission
Plasma-Derived Product Labeling Claims For TSE Clearance To Be Discussed By
Cmte.
FDA s Transmissible Spongiform Encephalopathies Advisory Committee will
discuss labeling claims for TSE clearance studies for plasma-derived products on
Oct. 14.
The committee will also receive updates on U.S. Department of Agriculture
licensed tests for the diagnosis of bovine spongiform encephalopathy, FDA BSE
food safety rules, and the BSE situation worldwide.
Presumptive transfusion transmissions of variant Creutzfeldt-Jakob Disease
and FDA recommended safeguards will also be addressed by the committee.
To watch a webcast of this meeting, click the button below. To arrange for
live videoconferencing, or to order videotapes & DVDs, email
webcasthelp@elsevier.com or call 800-627-8171.
Posted: Friday, September 10, 2004
Sign up to view this meeting.
October 14, 2004
------------------------------------------------------------------------
Previous Meetings Date Mad Cow Disease: FDA Recommendations To Minimize
Risk From TSE Agents In Medical Products -Day 2
February 13, 2004
------------------------------------------------------------------------
Mad Cow Disease: Risk Models For Bovine Sourcing For Medical Products;
First U.S. Case
February 12, 2004
------------------------------------------------------------------------
TSE Removal From Medical Devices and Equipment
July 18, 2003
------------------------------------------------------------------------
Bovine Bone Gelatin Safety; BSE In Canada
July 17, 2003
------------------------------------------------------------------------
Variant Creutzfeldt-Jakob Disease Guidance Implementation
February 20, 2003
------------------------------------------------------------------------
Blood Donor Deferral Implementation
June 27, 2002
------------------------------------------------------------------------
CJD Transmission Risk Reduction Draft Guidance
June 26, 2002
------------------------------------------------------------------------
TSE/Blood Products Joint Cmte. Meeting
January 17, 2002
------------------------------------------------------------------------
Creutzfeldt-Jakob Disease FDA Draft Guidance
October 25, 2001
------------------------------------------------------------------------
For information about additional meetings, email webcasthelp@elsevier.com
or call Julie D. Robenson at (800) 332-1370.
GREETINGS LIST MEMBERS,
talk about spooky. i was just going over a few old Advisory Committee
meetings;
FOOD AND DRUG ADMINISTRATION
NATIONAL INSTITUTES OF HEALTH
Advisory Committee on:
TRANSMISSIBLE
SPONGIFORM
ENCEPHALOPATHIES
December 18, 1998
Holiday Inn
8120 Wisconsin Avenue
Bethesda, Maryland
Proceedings by:
CASET Associates, Ltd
10201 Lee Highway, Suite 160
Fairfax, Virginia 22030
snip...
DR. ROOS: I have a question for Dr. Metters. I guess I just wanted to make
sure that I understood the rationale for the present policies in the United
Kingdom; that is that there is no pooled plasma products that have UK donors.
Nevertheless, there is no curtailment of blood transfusions and labile products
from UK individuals.
I just wanted to make sure I understood the rationale for that. That was
just -- in other words, if there is a safety problem with a particular unit
bearing CJD, then presumably those individual blood transfusions also carry that
risk. Maybe you could explain that.
DR. METTERS: First of all, all blood products. At the moment there are,
because we haven't completed the change-over from UK source to non-UK source.
Once that change-over takes place, there will be no UK sourced blood products.
We are making that change as soon as possible.
The reason why blood products may be different from blood is that, of
course, blood products go into an enormous pool.
The potential disbursement of a unit that is contaminated with new variant
depends on the size of the pool, whether it is a pool of 500 or 6,500 units.
As I said, we have to find an alternative source of blood products. The
most units it would go to is three recipients.
The other point to make is that this relates to the follow up. By far,
those who receive blood in the United Kingdom will die from the current disease
for which they receive the blood within 12 to 18 months. That is a real problem
when you come to holding it up, because of the attenuation and so on.
The blood products, that does present a disbursal factor. As I said, we do
actually have steps to monitor who receives the blood, and are taking steps to
out-source blood products.
DR. BROWN: Just to add, on the disbursal factor, we don't know what is
going on in new variant. We don't know if there are a million infectious units
per unit of blood. We just don't know.
I received this comment about CJD. If new variant is, indeed, like ordinary
CJD, there is a logical curiosity about the disposal factor. Infectivity is a
functional definition. We don't necessarily know what it is.
So, if there are 15 infectious units, we are talking about 15 transmissions
of the disease. It is likely, after all the experimental and epidemiologic
evidence that any level of infectivity in the blood of normal CJD is very, very
low.
It doesn't much matter if that 100 infectious units is distributed to
10,000 or a million. There are going to be 100 transmissions. The notion that
you can dilute out infectivity has no scientific basis.
The other thing, if the unit of blood that is donor is fractionated with an
infectious agent, then disbursal may be higher. Unfortunately, we don't know the
answer to that yet.
DR. SCHONBERGER: I am wondering if our colleagues from the United Kingdom
can tell us what type of screening for blood donors is done to reduce the
chances of new variant disease specifically in the donor group.
Is there any kind of screening specifically directed toward new variant
CJD.
The other issue, again, of screening, is there any screening that is done
in the United Kingdom that is focused specifically on ruling out somebody who is
symptomatic, for example, with new variant.
I understand that new variant's onset can be subtle and not really very
apparent for a while.
DR. BROWN: So, what you are asking is whether or not there are any special
criteria that are in place or being thought of to reduce the risk of a new
variant patient who is either -- according to Bob Will's criteria -- either
probable or definite. I can't imagine a definite, but shall we say a suspect.
DR. METTERS: I think the general answer has to be no. There is nothing you
can ask somebody. There is, on the other hand, the donor is at least asked about
their general health.
Then if there is any suspicion at the time that they are not 100 percent
fit, and they have their blood count done before they are accepted.
If someone is physically unwell in any way, hopefully they will not get
through the screening system. So, while it isn't specific to that, it is -- I
would be doing a very bad job if I let someone who was unhealthy in any way to
get through our screening system.
DR. SCHONBERGER: So, there is no set of questions that is standard --
DR. METTERS: The questions of about CJD are there, right. To avoid getting
classes of donors, you may be able to avoid getting classes of donors.
I would be very interested if any of you at the table could answer the
question that was asked.
We haven't yet had one who has been identified that in the time that they
were labile, was a donor.
DR. METTERS: As you know, most of the patients, or many of them, have
psychiatric onsets. So, if their response to the very first question you ask is
moo, you know to be suspicious.
DR. ROHWER: I have a question for Dr. Ferguson. When the provisions against
importations from the United Kingdom were extended in February of 1998, was
there any attempt by the USDA to go back to see what level of exposure the
United States had to European bovine products and cattle since 1980, for
example, or since 1988 when the provisions were put in place for the United
Kingdom?
For example, between 1980 and 1989, apparently we imported some 500 cattle.
Now that we have recognized that there may have also been a risk from imports
from Europe and others before this change in policy, have we gone back and
looked at how exposed we were from that risk? I mean, how many imports were
there, and what kind of things were imported.
DR. FERGUSON: Yes, actually we have gone back and looked at live animal
imports from continental Europe at that same time.
They were fairly restricted at that point in time because cattle in Europe
were infected with other animal diseases, such as FMB.
snip...
MR. SUDIERI(?): My name is Sal Sudieri. I am the vice president for medical
affairs at the New York Blood Center.
Regarding this section A, there is a piece of information that I think is
important for you to have.
For the last 25 years, the American Blood Center has had a program with
Switzerland, Holland and Germany, where centers that produce plasma derivatives
in this country collect units of whole blood from volunteer donors.
They became licensed centers, collection centers, from the New York Blood
Center, by our FDA license, and they will ship us the red cells, where we do the
processing, dedicate the plasma and the plasma is fractionated.
About 30 percent of the blood, or about 200,000 units of red cells a year,
come to New York through this method.
DR. BROWN: Is the committee happy about what everybody considers and knows
to be an other BSE country or do we want to get clarification of that?
DR. LEITMAN: Clarification.
DR. BROWN: Are we talking about the United Kingdom plus France, plus
Portugal, plus the whole of Europe? What are we talking about?
DR. LURIE: Who is to make that decision?
DR. BROWN: That is what we want to know. Is that a decision that is going
to be made? If they can't tell us what is meant by other BSE country, we can't
really answer the question.
DR. LURIE: The procedural approach would be to vote on it separately. I
think the vote is more providing guidance.
DR. BROWN: Good suggestion. Ditto for periods of higher and lower risk, I
suppose, and ditto for possible versus probable. We can move along in that way.
That is a good idea.
DR. LURIE: Another parallel type suggestion would be, I think the question
that we do need some clarification on is the definition of reside.
While obviously it is more efficient to exclude residents from Britain and
visitors from Britain because, a, there are presumably fewer residents than
there are visitors, and the duration of exposure and presumably severity of
exposure would be different.
DR. BROWN: Maybe the best way to do it is to go piecemeal and nibble, in
which case we might, for example, phrase the first question, should the FDA
recommend excluding donors who are British citizens and see what you get in
answer to that, and see just how far the committee is willing to go.
On the other hand, that is going to require about 117 votes this afternoon.
DR. HOEL: There is another approach. First, we have to answer the first
question first.
DR. BROWN: I know. That was going to be my next point. Depending on our
answer to one, we can either dismiss A through E or take them up. I think that
is why Dr. Epstein phrased these two questions in this way. Maybe I am wrong,
but that is the way it is going to be done.
If the committee is ready to vote without further discussion on question
one -- not A, B, C, D and E, but just question one as a question -- we will then
vote and see what we then have to do, or we can have a little, a moderate or a
large amount of discussion before we get to that.
DR. LEITMAN: I have always had a problem with reducing a theoretical risk
or reducing a hypothetical risk or reducing a potential risk, because perhaps,
as I was talking to one of my colleagues earlier today, perhaps it is a
speculative risk and not a theoretical risk that, in actuality, hasn't occurred.
How do we reduce speculative risk? How do you reduce zero?
DR. BROWN: That is an interesting kind of semantic question. It is the
virtual reduction of a theoretical risk. Does anyone want to get into semantics?
DR. CLIVER: Clearly question one turns on the perception of nvCJD as a food
borne disease that is somehow derived from cattle.
I think I am prepared to accept that. The period of emphasis ending at
1990, though, I think is not indicated. The observations that Dr. Detweiler had
before, the data on this would have been very valuable for risk on people on
farms, however, there is no imputation here that the risk was associated with
people on farms with cattle.
[try 15 times more likely to get CJD...TSS]
CONFIRMATION OF CJD IN FOURTH FARMER
now story changes from;
SEAC concluded that, if the fourth case were confirmed, it would be
worrying, especially as all four farmers with CJD would have had BSE cases on
their farms.
to;
This is not unexpected...
was another farmer expected?
4th farmer, and 1st teenager
snip...
DR. BROWN: The question, Robert, is there any instance of maternal
transmission of new variant CJD?
DR. WILL: The answer is no, although tragically, at least one of the
individuals was pregnant when diagnosed with new variant CJD.
MS. HARRELL: Was she delivered?
DR. WILL: She did delivered. The child is alive and well, but we are only
talking two or three years. Of course, therefore, we rely on previous evidence,
which does not suggest that there was maternal transmission.
DR. BROWN: It is an interesting question. If it were to have occurred, it
would be one more very striking example of a particular biological behavior of
new variant from sporadic.
We have information about half a dozen children born to patients who were
sick with CJD on delivery, who now have -- they have lived for as long as 30
years after that event -- that is, after they were born -- and are quite
healthy...
snip...
DON'T count your chickens again before they hatch there Dr. Brown, remember
the 38 years incubation period on the iCJD of a small dose;
SHORT REPORT
Creutzfeldt-Jakob disease 38 years after diagnostic use of human growth
hormone
E A Croes, G Roks, G H Jansen, P C G Nijssen, C M van Duijn
...............................................................
J Neurol Neurosurg Psychiatry 2002;72:792-793
We describe the second patient with hGH related CJD in the Netherlands. The
patient developed the disease 38 years after hGH injections. To our knowledge,
this is the longest incubation period described for any form of iatrogentic CJD.
Furthermore, our patient was _not_ treated with hGH, but only received a _low_
dose as part of a diagnostic procedure...
and this was not a case of nv/v CJD, but spordic CJD...TSS
ACTION TAKEN BY THE UK MEDICINES CONTROL AGENCY ON BOVINE INGREDIENTS IN
MEDICINAL PRODUCTS
6.1 Information is held on a especial data base from the initial BSE survey
in 1989. More current products, which were vetted during their initial licensing
assessment, after the UK BSE quideline was published, may not appear in the
listing, since BSE was considering in their initial licensing assessment.
RESTRICTED-COMMERCIAL..............CSM/BIOLS/94/6th Meeting
NOT FOR PUBLICATION
COMMITTEE ON SAFETY OF MEDICINES
SUB-COMMITTEE ON BIOLOGICALS
snip...
1.1 The chairman reminded the Sub-Committee that the papers and proceedings
were confidential and should not be disclosed...
snip...
4.6 BOVINE SERA
4.6.1 There was a discussion of the problem of the paper trail audit, and
countries of sourcing for bovine sera. Concern was expressed about the fact that
bogus certificates of origin had been produced and circulated. It was noted that
in the USA bovine protein was still being fed to cattle. Sera from the USA could
be used in the initial production and subsequent large scale manufacture of
hybridomas, rDNA products and vaccines (some of which may be used in healthy
children)...
RESTRICTED-COMMERCIAL CSM/94/8th Meeting
NOT FOR PUBLICATION
COMMITTEE ON SAFETY OF MEDICINES
(7 blank pages to follow...TSS)
7.4 The Committee was informed that, since the publication of Hunter et al.
(2002), two further sheep had succumbed to BSE; one of these sheep had been
transfused with buffy coat, while the other had been transfused with whole
blood. Of the remaining 20 sheep that received transfusions, one died of
unrelated causes and 19 animals remain apparently healthy. The healthy animals
are at varying times post-transfusion ranging from less than 100 to over 1000
days.
7.5 Members were informed that among the 21 sheep transfused with blood
from scrapie infected animals (761 to 1080 days of age), 4 had developed scrapie
between 614 and 737 days post-transfusion. One animal received the buffy coat
preparation from the blood of an animal with clinical disease. The remaining 3
animals received whole blood from donors not yet showing clinical signs. Of the
remaining 17 transfused sheep, one died of unrelated causes and two further
sheep were showing clinical signs of scrapie; one of these had received buffy
coat, while the other had received whole blood.
7.6 The Committee noted that it would not be possible to confirm that the
negative controls are free of TSEs until the end of the study, when they would
be culled and analysed post mortem for signs of subclinical infection. Of the 10
positive controls that received BSE-infected cattle brain homogenate
intravenously, 5 have developed disease or appear to be in the early stages of
the disease.
3 Hunter, N., Foster, J., Chong, A., McCutcheon, S., Parnham, D., Eaton,
S., MacKenzie, C. and Houston, F. (2002). Transmission of prion disease by blood
transfusion. Journal of General Virology 83.
www.socgenmicrobiol.org.uk/JGVDirect/18580/18580ft.pdf
CJD: Transmission
Lord Lucas asked Her Majesty's Government:
Further to the Written Answer by Lord Hunt of Kings Heath on 27 March (WA
59), what is the "strong epidemiological evidence to suggest that classic CJD is
not transmitted through blood"; which of the many variants of "classic CJD" this
evidence applies to; and whether they will place copies of the relevant papers
in the Library of the House.[HL1864]
The Parliamentary Under-Secretary of State, Department of Health (Lord Hunt
of Kings Heath): The following, peer reviewed articles, relate to this subject
and will be placed in the Library:
T F G Esmonde et al, 1993, Creutzfeldt-Jakob disease and blood transfusion,
Lancet 341; 205-207;
P Brown, 1995, Can Creutzfeldt-Jakob disease be transmitted by
transfusion?, Current Opinion in Haematology, vol 2, pp 472-477;
C M van Duijn et al, 1998, Case-control study of risk factors of
Creutzfeldt-Jakob disease in Europe during 1993-95, Lancet 351: 1081-1085;
11 Apr 2000 : Column WA32
Ricketts MN et al: Is Creutzfeldt-Jakob Disease transmitted in blood?,
Energ Infect Dids 1997:3, 155-166; and
Heye N et al: Creutzfeldt-Jakob Disease and blood transfusion, Lancet 1994;
343; 298-299.
These studies cover all types of CJD. Sporadic (classic) CJD, however,
accounts for some 85 per cent of non-variant cases.
The European Committee for Proprietory Medicinal Products (CPMP) reviewed
the evidence in December 1995 and advised that there was no experimental or
epidemiological evidence that classical CJD is transmitted by blood transfusions
or plasma-derived products. A recall policy was not considered justified for
plasma derived products from plasma pools incorporating a donation implicated
for classical CJD. It reaffirmed that advice in March 1997. CPMP concluded on
the basis of currently available information from epidemiological and
experimental studies that there is no scientific justification for changing from
the current CPMP position on classical CJD. CPMP further stated there to be no
evidence that classical CJD is transmitted via blood or plasma derived products.
This issue was also subsequently considered by the US Food and Drugs
Administration, which came to the same conclusion.
New-variant CJD
Lord Lucas asked Her Majesty's Government:
Further to the Written Answer by Lord Hunt of Kings Heath on 27 March (WA
55-6), whether they will provide data on those who have died from, or been
diagnosed with, new-variant CJD in the United Kingdom as to:
(a) the status of nucleotide-21 preceding the prion ATG start codon;
(b) the status of codons 26, 56 and 174 of doppel;
(c) the dates of onset and confirmation for those patients diagnosed with
new-variant CJD but still living;
(d) the definition of "onset"; and
(e) the age of the patients at onset to the nearest month.[HL1807]
Lord Hunt of Kings Heath: The genetic information requested is not
available. However, extensive studies of polymorphisms in and around the prion
protein and doppel genes have been under way for some time at the St Mary's
Prion Unit, London. The results of these investigations will be published in the
scientific literature, subject to peer review, in due course.
Confirmation of a diagnosis of vCJD is currently obtained by postmortem
neuropathology. There are therefore no "confirmed" patients still living. The
dates of onset for patients still living and defined as "probable" to the
nearest month are as follows:
25 Apr 2002 : Column WA58
Bovine Embryos and Live Cattle: Imports from North America
The Earl of Caithness asked her Majesty's Government:
When the ban on the importation of embryos and live cattle from North
America will be lifted; and [HL3912]
What is the scientific evidence for the imposition of a ban on the
importation of embryos and live cattle from North America. [HL3913]
Lord Whitty: Her Majesty's Government have not imposed a ban on imports of
bovine embryos and live cattle from North America.
The European Parliament and European Council introduced legislation in May
last year laying down rules for the prevention, control and eradication of
certain transmissible spongiform encephalopathies (TSEs). The legislation was
introduced in response to the recommendations of the Office International des
Epizooties (OIE the international animal health organisation) and advice from
the Commission's scientific comittees. The legislation (and the transitional
measures which came into effect in October last year) includes requirement that
imports into the EU of bovine embryos and live cattle must be accompanied by
certification confirming that the feeding of ruminants with protein derived from
mammals has been banned and that the ban has been effectively enforced. Some
exporting countries, such as Canada and the USA, are currently unable to meet
these new requirements.
BSE: US Export of Specified Risk Material
Lord Kennet asked Her Majesty's Government:
Whether the United States contends that under the provisions enforceable by
the World Trade Organisation the European Union may not ban the import into
Europe from the United States of "specified risk material" (that is, material at
possible risk of BSE infection).
Lord Donoughue: Yes. But their position on the Specified Risk Material
legislation is based on the assumption that the United States can safely be
regarded as a "BSE free" country. Their case for such treatment has not been
accepted by the EU Commission's Scientific Veterinary Committee.
Baroness Masham of Ilton: My Lords, as blood products which infected
haemophiliacs with HIV came from the USA, is the Minister confident that
something else nasty may not come again from imported blood from the USA? Is he
aware that there are ways of cleaning blood to make it safer? I know that that
is done in Vienna, in Austria. Will the Minister look into that? Following the
question asked by the noble Lord, Lord Clement-Jones, about people using their
own blood, I am sure that, when this Statement goes out into the wider
community, people will want to know that information.
However, the Bio Products Laboratory who produce plasma products did export
surplus products, under the Income Generation Regulations for the NHS, and used
the income for the benefit of the health service.[21]
43. Do you sell any of it abroad at all?
(Mr Gorham) No. The only circumstances in which we would export blood would
be if there was an approach to the British Government and the British Government
felt that it was appropriate to support an international emergency or something
like that. We do supply the British Forces. We occasionally help out our
colleagues in Wales and Scotland and they would reciprocate with us if that was
appropriate. At the moment it is more or less totally contained within the
United Kingdom.
Blood and Blood Products
Mr. Hinchliffe: To ask the Secretary of State for Health what estimate he
has made of the number of persons who have been inoculated with blood or blood
products over the past three years in the United Kingdom. [61681]
Ms Jowell [holding answer 2 December 1998]: It is estimated that about one
million people in the United Kingdom receive blood and blood products every
year.
CJD blood products given to 3,000 patients
December 16 1997 BY AUDREY MAGEE AND IAN MURRAY
UP TO 3,000 people treated in 100 British hospitals may have been injected
with blood products taken from a donor who died six weeks ago from new variant
Creutzfeldt-Jakob disease, the human form of BSE. None of them is to be told
because the Health Department believes the risk of them developing the disease
is so slight that there is no reason to cause alarm.
Although hospitals have been advised to return the product, used in X-ray
screenings to detect lung disease, so far only 15 per cent has been recovered.
There is no order obliging hospitals to return it and some clinicians may go on
using up stocks on the basis that patients are far more likely to die from
infections or cancer that can be diagnosed with the product than from CJD.
Another 268 patients in Ireland are known to have been given injections
from the same batch of the product. The Irish Health Ministry has decided to
notify all the patients concerned.
Even though the identity of all those who have been given an injection of
the product is known, it was decided not to tell them because there is no
evidence that the illness can be transmitted through the blood or the serum
derived from it to make the product and the risk of developing CJD is regarded
as negligible.
"You are putting an enormous burden on people by telling them they have a
remote risk of contracting the disease," the department said last night. "The
ethics committee which advises us on these matters decided it was just not
appropriate to tell them."
The blood from the donor was sent 18 months ago to the National Blood
Authority laboratory, where it was split into a number of different products.
The donor's plasma was mixed with some taken from 49,000 other donors to make
8,174 bottles of albumin, the water-soluble protein found in blood.
Many were exported but 210 of the 50ml bottles remained in Britain and were
sent to eight different hospitals and companies. Some of the bottles were used
intravenously to rehydrate burn victims.
One bottle was sent to Nycomed Amersham which used it to produce 14,000
vials of Amerscan Pulmonate II, an agent which is injected into the lungs so
that infections and cancer show up under X-ray. The company sent almost 3,700
vials to 100 British hospitals.
At the end of October the European Committee on Proprietary Medicinal
Products called for the withdrawal of blood products derived from donors who
were confirmed CJD cases. On November 1 the Blood Transfusion Service was
notified that one of its donors had died from the disease so the Amersham
company was told.
In turn the company got in touch with the Medicines Control Agency which
informed the Health Department and it recommended withdrawal of the product on
November 17.
Despite regular alarms, there has never been any convincing evidence that
blood or blood products can transmit CJD (Nigel Hawkes writes). Unless new
variant CJD, the human form of "mad cow" disease, is more easily transmitted
than classic CJD via blood or blood products, there does not appear to be any
cause for concern. [This is no medical basis for this statement -- webmaster]
For classic CJD the risk seems negligible. About 50 people a year die of
the disease, so it is certain that every year some of them give blood after they
have the infection but before its symptoms appear. Studies show that classic CJD
can be passed on in human tissue, but not - so far as we know - in blood. [This
is not an accurate summary of current scientific knowledge -- webmaster]
South Africa has been exporting plasma for many years. From 1983-86, human
plasma was falsely labelled "animal plasma" and illegally exported to Europe.
This illegal practice resulted in a court case and one conviction in Belgium. In
1996, Austrian police seized 4000 L of infected blood from a Linz-based company,
Albovina.
The Sunday Times report said that Austrian officials have investigated two
British companies, based in Guernsey and Berkshire. Police are still trying to
find out where the companies' plasma products were used. Johann Kurz, head of
unit for biologicals with the federal Ministry of Social Security &
Generation in Vienna, Austria, told The Lancet, "We suspect that in 1996 and
earlier, some products coming from South Africa were transferred to India,
China, and Hong Kong, among other countries". The products were albumin and
intravenous immunoglobulins.
Nevertheless, Luc Noel, Coordinator of Blood Safety with the WHO in Geneva,
Switzerland, emphasised that: "There should not be any confusion of the
transfusion services in South Africa--which are now world class--with these
criminal activities". While it is important that this trafficking is exposed,
the public can be confident in their blood services, Noel added.
Sanjay Kumar
Kirsten Myhr, MScPharm, MPH Bygdøy alle 58B 0265 Oslo, Norway Tel.: +47 22
56 05 85, fax: +47 22 24 90 17 myhr@online.no
TSS
Thursday, September 10, 2015
25th Meeting of the Transmissible Spongiform Encephalopathies Advisory
Committee Food and Drug Administration Silver Spring, Maryland June 1, 2015
Sunday, December 14, 2014
*** ALERT new variant Creutzfeldt Jakob Disease nvCJD or vCJD, sporadic CJD
strains, TSE prion aka Mad Cow Disease United States of America Update December
14, 2014 Report ***
Sunday, March 09, 2014
A Creutzfeldt-Jakob Disease (CJD) Lookback Study: Assessing the Risk of
Blood Borne Transmission of Classic Forms of Creutzfeldt-Jakob Disease
FDA TSEAC CIRCUS AND TRAVELING ROAD SHOW FOR THE TSE PRION DISEASES
Please send your reply to the Food and Drug Administration, Attention: Tyra
S. Wisecup, Compliance Officer, at the address on the letterhead. If you have
questions regarding any issues in this letter, please contact Ms. Wisecup at
(612) 758-7114. Sincerely, /S/ Michael Dutcher, DVM Director Minneapolis
District
--------------------------------------------------------------------------------
[1] Specified risk materials include the brain, skull, eyes, trigeminal
ganglia, spinal cord, vertebral column (excluding the vertebrae of the tail, the
transverse processes of the thoracic and lumbar vertebrae, and the wings of the
sacrum), and dorsal root ganglia of cattle 30 months of age and older, and the
tonsils and distal ileum of the small intestine of all cattle. - Page Last
Updated: 11/15/2012
I made a submission to the BSE Inquiry long ago during the BSE Inquiry
days, and they seemed pretty interested. see on down ;
Sender: "Patricia Cantos"
To: "Terry S Singeltary Sr. (E-mail)"
Subject: Your submission to the Inquiry
Date: Fri, 3 Jul 1998 10:10:05 +0100
3 July 1998
Mr Terry S Singeltary Sr.
E-Mail: Flounder at wt.net
Ref: E2979
Dear Mr Singeltary,
Thank you for your E-mail message of the 30th of June 1998 providing the
Inquiry with your further comments.
Thank you for offering to provide the Inquiry with any test results on the
nutritional supplements your mother was taking before she died.
As requested I am sending you our general Information Pack and a copy of
the Chairman's letter. Please contact me if your system cannot read the
attachments.
Regarding your question, the Inquiry is looking into many aspects of the
scientific evidence on BSE and nvCJD. I would refer you to the transcripts of
evidence we have already heard which are found on our internet site at ;
Could you please provide the Inquiry with a copy of the press article you
refer to in your e-mail? If not an approximate date for the article so that we
can locate it?
In the meantime, thank you for you comments. Please do not hesitate to
contact me on...
snip...end...tss
everyone I tell this too gets it screwed up...MY MOTHER WAS NOT TAKING
THOSE SUPPLEMENTS IPLEX (that I ever knew of). this was my neighbors mother that
died exactly one year _previously_ and to the day of sporadic CJD that was
diagnosed as Alzheimer’s at first. my mother died exactly a year later from the
Heidenhain Variant of Creutzfeldt Jakob Disease hvCJD, and exceedingly rare
strains of the ever growing sporadic CJD’s. _both_ cases confirmed. ...kind
regards, terry
TSEs i.e. mad cow disease's BSE/BASE and NUTRITIONAL SUPPLEMENTS
IPLEX, mad by standard process;
vacuum dried bovine BRAIN, bone meal, bovine EYE, veal Bone, bovine liver
powder, bovine adrenal, vacuum dried bovine kidney, and vacuum dried porcine
stomach.
also;
what about potential mad cow candy bars ?
see their potential mad cow candy bar list too...
THESE are just a few of MANY of just this ONE COMPANY...TSS
DEPARTMENT OF HEALTH AND HUMAN SERVICES
FOOD AND DRUG ADMINISTRATION CENTER FOR BIOLOGICS EVALUATION AND RESEARCH
TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES ADVISORY COMMITTEE
Friday, January 19, 2001 snip...
17 But I think that we could exhibit some quite
18 reasonable concern about blood donors who are taking dietary
19 supplements that contain a certain amount of unspecified-
20 origin brain, brain-related, brain and pituitary material.
21 If they have done this for more than a sniff or something
22 like that, then, perhaps, they should be deferred as blood
23 donors.
24 That is probably worse than spending six months in
25 the U.K.
1/19/01
3681t2.rtf(845) page 501
Docket Management Docket: 96N-0417 - Current Good Manufacturing Practice in
Manufacturing, Packing, or Holding Dietary Ingredients a Comment Number: EC -2
Accepted - Volume 7
see full text ;
snip...see full list of potential mad cow products ;
Wednesday, August 5, 2015
Federal judge enters permanent injunction against Wisconsin dietary
supplement manufacturers prohibited cattle materials BSE TSE Prion
Singeltary Submission to BSE Inquiry on Nutritional Supplements containing
SRM 1998
Tue, 13 Feb 2001 JAMA Vol. 285 No. 6, February 14, 2001 Letters
Diagnosis and Reporting of Creutzfeldt-Jakob Disease � � To the Editor:
In their Research Letter in JAMA. 2000;284:2322-2323, Dr Gibbons and
colleagues1 reported that the annual US death rate due to Creutzfeldt-Jakob
disease (CJD) has been stable since 1985. These estimates, however, are based
only on reported cases, and do not include misdiagnosed or preclinical cases. It
seems to me that misdiagnosis alone would drastically change these figures. An
unknown number of persons with a diagnosis of Alzheimer disease in fact may have
CJD, although only a small number of these patients receive the postmortem
examination necessary to make this diagnosis. Furthermore, only a few states
have made CJD reportable. Human and animal transmissible spongiform
encephalopathies should be reportable nationwide and internationally.
Terry S. Singeltary, Sr Bacliff, Tex
To the Editor:
At the time of my mother's death, various diagnoses were advanced such as
"rapid progressive Alzheimer disease," psychosis, and dementia. Had I not
persisted and personally sought and arranged a brain autopsy, her death
certificate would have read cardiac failure and not CJD.
Through CJD Voice1 I have corresponded with hundreds of grief-stricken
families who are so devastated by this horrific disease that brain autopsy is
the furthest thing from their minds. In my experience, very few physicians
suggest it to the family. After the death and when families reflect that they
never were sure what killed their loved one it is too late to find the true
cause of death. In the years since my mother died I think that the increasing
awareness of the nature of CJD has only resulted in fewer pathologists being
willing to perform an autopsy in a suspected case of CJD.
People with CJD may die with incorrect diagnoses of dementia, psychosis,
Alzheimer disease, and myriad other neurological diseases. The true cause of
death will only be known if brain autopsies are suggested to the families. Too
often the physician's comment is, "Well, it could be CJD but that is so rare it
isn't likely."
Until CJD is required to be reported to state health departments, as other
diseases are, there will be no accurate count of CJD deaths in the United States
and thus no way to know if the number of deaths is decreasing, stable, or
increasing as it has recently in the United Kingdom.
Dorothy E. Kraemer Stillwater, Okla
In Reply:
Mr Singeltary and Ms Kraemer express an underlying concern that our
recently reported mortality surveillance estimate of about 1 CJD case per
million population per year in the United States since 1985 may greatly
underestimate the true incidence of this disease. Based on evidence from
epidemiologic investigations both within and outside the United States, we
believe that these national estimates are reasonably accurate.
Even during the 1990s in the United Kingdom, where much attention and
public health resources have been devoted to prion disease surveillance, the
reported incidence of classic CJD is similar to that reported in the United
States. [The elderly demented in a country with a medical system like England's
rarely reach a neurologist. However, it is precisely the elderly where the
disease is concentrated. -- webmaster]
In addition, in 1996, active US surveillance for CJD and new variant (nv)
CJD in 5 sites detected no evidence of the occurrence of nvCJD and showed that
86% of the CJD cases in these sites were identifiable through routinely
collected mortality data. [This again was merely "death certificate" CJD. --
webmaster]
Our report provides additional evidence against the occurrence of nvCJD in
the United States based on national mortality data analyses and enhanced
surveillance. It specifically mentions a new center for improved pathology
surveillance. We hope that the described enhancements along with the
observations of Singeltary and Kraemer will encourage medical care providers to
suggest brain autopsies for more suspected CJD cases to facilitate the
identification of potentially misdiagnosed CJD cases and to help monitor the
possible occurrence of nvCJD.
Creutzfeldt-Jakob disease is not on the list of nationally notifiable
diseases. In those states where surveillance personnel indicate that making this
disease officially notifiable would meaningfully facilitate collection of data
that are needed to monitor the incidence of CJD and nvCJD, including the
obtaining of brain autopsy results, we encourage such a change. However, adding
CJD to the notifiable diseases surveillance system may lead to potentially
wasteful, duplicative reporting because the vast majority of the diagnosed cases
would also be reported through the mortality surveillance system.
Furthermore, making CJD a notifiable disease may not necessarily help
identify undiagnosed CJD cases. The unique characteristics of CJD make mortality
data a useful surrogate for ongoing surveillance. Unlike many other neurologic
diseases, CJD is invariably fatal and in most cases rapidly progressive and
distinguishable clinically from other neurologic diseases. [Essentially all
elderly dementia is "fatal" in the sense that no one gets better. -- webmaster]
Because CJD is least accurately diagnosed early in the course of the
illness, notifiable disease surveillance of CJD could be less accurate than
mortality surveillance of CJD. In addition, because death as a condition is more
completely and consistently reported, mortality surveillance has the advantage
of being ongoing and readily available.
The absence of CJD and nvCJD from the list of nationally notifiable
diseases should not be interpreted to mean that they are not important to public
health; this list does not include all such diseases. We encourage medical
caregivers to report to or consult with appropriate public health authorities
about any diagnosed case of a transmissible disease for which a special public
health response may be needed, including nvCJD, and any patient in whom
iatrogenic transmission of CJD may be suspected.
Robert V. Gibbons, MD, MPH Robert C. Holman, MS Ermias D. Belay, MD
Lawrence B. Schonberger, MD, MPH Division of Viral and Rickettsial Diseases
National Center for Infectious Diseases Centers for Disease Control and
Prevention Atlanta, Ga
Opinion (webmaster): Behind the polite words, CDC is waging a bitter battle
to prevent CJD incidences from becoming known, to protect its clients in the
blood and transplant industries. The best available study is that of Boller et
al. from 1989. In this study 7.5% of Alzheimer patients were actually CJD.
Numerous other diseases were confused as well.
With CJD, the real issue is how many people die with it, rather from it in
the sense of the death certificate. Even if the true cause of death was a heart
attack, as a hypothetical, who would want an untested cornea from someone with
preclinical CJD ?
Diagnosis of dementia: clinicopathologic correlations. Neurology 1989
Jan;39(1):76-79 Boller F, Lopez OL, Moossy J Based on 54 demented patients
consecutively autopsied at the University of Pittsburgh, we studied the accuracy
of clinicians in predicting the pathologic diagnosis. Thirty-nine patients
(72.2%) had Alzheimer's disease, while 15 (27.7%) had other CNS diseases (four
multi-infarct dementia; three Creutzfeldt-Jakob disease; two thalamic and
subcortical gliosis; three Parkinson's disease; one progressive supranuclear
palsy; one Huntington's disease; and one unclassified). Two neurologists
independently reviewed the clinical records of each patient without knowledge of
the patient's identity or clinical or pathologic diagnoses; each clinician
reached a clinical diagnosis based on criteria derived from those of the
NINCDS/ADRDA. In 34 (63%) cases both clinicians were correct, in nine (17%) one
was correct, and in 11 (20%) neither was correct. These results show that in
patients with a clinical diagnosis of dementia, the etiology cannot be
accurately predicted during life.
26 March 2003
Views & Reviews
Monitoring the occurrence of emerging forms of Creutzfeldt-Jakob disease in
the United States
Ermias D. Belay, MD, Ryan A. Maddox, MPH, Pierluigi Gambetti, MD and
Lawrence B. Schonberger, MD
+ Author Affiliations
From the Division of Viral and Rickettsial Diseases (Drs. Belay and
Schonberger and R.A. Maddox), National Center for Infectious Diseases, Centers
for Disease Control and Prevention, Atlanta, GA; and National Prion Disease
Pathology Surveillance Center (Dr. Gambetti), Division of Neuropathology,
Institute of Pathology, Case Western Reserve University, Cleveland, OH.
Address correspondence and reprint requests to Dr. Ermias D. Belay, 1600
Clifton Road, Mailstop A-39, Atlanta, GA 30333.
26 March 2003
Terry S. Singeltary, retired (medically) CJD WATCH
I lost my mother to hvCJD (Heidenhain Variant CJD). I would like to comment
on the CDC's attempts to monitor the occurrence of emerging forms of CJD.
Asante, Collinge et al [1] have reported that BSE transmission to the
129-methionine genotype can lead to an alternate phenotype that is
indistinguishable from type 2 PrPSc, the commonest sporadic CJD. However, CJD
and all human TSEs are not reportable nationally. CJD and all human TSEs must be
made reportable in every state and internationally. I hope that the CDC does not
continue to expect us to still believe that the 85%+ of all CJD cases which are
sporadic are all spontaneous, without route/source. We have many TSEs in the USA
in both animal and man. CWD in deer/elk is spreading rapidly and CWD does
transmit to mink, ferret, cattle, and squirrel monkey by intracerebral
inoculation. With the known incubation periods in other TSEs, oral transmission
studies of CWD may take much longer. Every victim/family of CJD/TSEs should be
asked about route and source of this agent. To prolong this will only spread the
agent and needlessly expose others. In light of the findings of Asante and
Collinge et al, there should be drastic measures to safeguard the medical and
surgical arena from sporadic CJDs and all human TSEs. I only ponder how many
sporadic CJDs in the USA are type 2 PrPSc?
The Pathological Protein:
Mad Cow, Chronic Wasting, and Other Deadly Prion Diseases
Philip Yam
''Answering critics like Terry Singeltary, who feels that the US
undercounts CJD, Schonberger _conceded_ that the current surveillance system has
errors but stated that most of the errors will be confined to the older
population''....end
2015
Clinically Unsuspected Prion Disease Among Patients With Dementia Diagnoses
in an Alzheimer’s Disease Database
Ryan A. Maddox, PhD1⇑ J. L. Blase, MPH1 N. D. Mercaldo, MS2,3 A. R. Harvey,
MSPH1 L. B. Schonberger, MD1 W. A. Kukull, PhD3 E. D. Belay, MD1 1Division of
High-Consequence Pathogens and Pathology, National Center for Emerging and
Zoonotic Infectious Diseases, Centers for Disease Control and Prevention,
Atlanta, GA, USA 2Department of Biostatistics, Vanderbilt University, Nashville,
TN, USA 3National Alzheimer’s Coordinating Center, University of Washington,
Seattle, WA, USA Ryan A. Maddox, PhD, Centers for Disease Control and
Prevention, 1600 Clifton Road, Mailstop A-30, Atlanta, GA 30333, USA. Email:
rmaddox@cdc.gov
Abstract Background: Brain tissue analysis is necessary to confirm prion
diseases. Clinically unsuspected cases may be identified through neuropathologic
testing.
Methods: National Alzheimer’s Coordinating Center (NACC) Minimum and
Neuropathologic Data Set for 1984 to 2005 were reviewed. Eligible patients had
dementia, underwent autopsy, had available neuropathologic data, belonged to a
currently funded Alzheimer’s Disease Center (ADC), and were coded as having an
Alzheimer’s disease clinical diagnosis or a nonprion disease etiology. For the
eligible patients with neuropathology indicating prion disease, further clinical
information, collected from the reporting ADC, determined whether prion disease
was considered before autopsy.
Results: Of 6000 eligible patients in the NACC database, 7 (0.12%) were
clinically unsuspected but autopsy-confirmed prion disease cases.
Conclusion: The proportion of patients with dementia with clinically
unrecognized but autopsy-confirmed prion disease was small. Besides confirming
clinically suspected cases, neuropathology is useful to identify unsuspected
clinically atypical cases of prion disease.
prion disease Creutzfeldt–Jakob disease Alzheimer’s disease dementia
diagnosis
> Results: Of 6000 eligible patients in the NACC database, 7 (0.12%)
were clinically unsuspected but autopsy-confirmed prion disease cases.
those figures seem large... tss
***Miracles do happen. it just took 3 decades of denial from these authors
cdc et al to finally come up with this conclusion, during that time period how
many humans have been exposed due to their continued denial over those decades?
see full text with old studies confirming misdiagnosis figures over the
decades...tss Wednesday, September 2, 2015
Clinically Unsuspected Prion Disease Among Patients With Dementia Diagnoses
in an Alzheimer’s Disease Database
Thursday, August 13, 2015
Iatrogenic CJD due to pituitary-derived growth hormone with genetically
determined incubation times of up to 40 years
Self-Propagative Replication of Ab Oligomers Suggests Potential
Transmissibility in Alzheimer Disease
Received July 24, 2014; Accepted September 16, 2014; Published November 3,
2014
*** Singeltary comment ***
Thursday, October 1, 2015
Alzheimergate, re-Evidence for human transmission of amyloid-β pathology
and cerebral amyloid angiopathy, Singeltary Submission to Nature
Thursday, November 12, 2015
Evaluation of the protection of primates transfused with variant
Creutzfeldt-Jakob disease–infected blood products filtered with prion removal
devices: a 5-year update
2 January 2000
British Medical Journal
U.S. Scientist should be concerned with a CJD epidemic in the U.S., as well
15 November 1999
British Medical Journal
vCJD in the USA * BSE in U.S.
The Lancet Infectious Diseases, Volume 3, Issue 8, Page 463, August 2003
doi:10.1016/S1473-3099(03)00715-1Cite or Link Using DOI
Tracking spongiform encephalopathies in North America
Original
Xavier Bosch
“My name is Terry S Singeltary Sr, and I live in Bacliff, Texas. I lost my
mom to hvCJD (Heidenhain variant CJD) and have been searching for answers ever
since. What I have found is that we have not been told the truth. CWD in deer
and elk is a small portion of a much bigger problem.” 49-year—old Singeltary is
one of a number of people who have remained largely unsatisfied after being told
that a close relative died from a rapidly progressive dementia compatible with
spontaneous Creutzfeldt—Jakob ...
Suspect symptoms
What if you can catch old-fashioned CJD by eating meat from a sheep
infected with scrapie?
28 Mar 01
Most doctors believe that sCJD is caused by a prion protein deforming by
chance into a killer. But Singeltary thinks otherwise. He is one of a number of
campaigners who say that some sCJD, like the variant CJD related to BSE, is
caused by eating meat from infected animals. Their suspicions have focused on
sheep carrying scrapie, a BSE-like disease that is widespread in flocks across
Europe and North America. Now scientists in France have stumbled across new
evidence that adds weight to the campaigners' fears. To their complete surprise,
the researchers found that one strain of scrapie causes the same brain damage in
mice as sCJD.
"This means we cannot rule out that at least some sCJD may be caused by
some strains of scrapie," says team member Jean-Philippe Deslys of the French
Atomic Energy Commission's medical research laboratory in Fontenay-aux-Roses,
south-west of Paris. Hans Kretschmar of the University of Göttingen, who
coordinates CJD surveillance in Germany, is so concerned by the findings that he
now wants to trawl back through past sCJD cases to see if any might have been
caused by eating infected mutton or lamb...
cwd to humans, iatrogenic, what if ?
Transmission of Creutzfeldt-Jakob disease to a chimpanzee by electrodes
contaminated during neurosurgery.
Gibbs CJ Jr, Asher DM, Kobrine A, Amyx HL, Sulima MP, Gajdusek DC.
Laboratory of Central Nervous System Studies, National Institute of Neurological
Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Stereotactic multicontact electrodes used to probe the cerebral cortex of a
middle aged woman with progressive dementia were previously implicated in the
accidental transmission of Creutzfeldt-Jakob disease (CJD) to two younger
patients. The diagnoses of CJD have been confirmed for all three cases. More
than two years after their last use in humans, after three cleanings and
repeated sterilisation in ethanol and formaldehyde vapour, the electrodes were
implanted in the cortex of a chimpanzee. Eighteen months later the animal became
ill with CJD. This finding serves to re-emphasise the potential danger posed by
reuse of instruments contaminated with the agents of spongiform
encephalopathies, even after scrupulous attempts to clean them.
Tuesday, May 26, 2015
*** Minimise transmission risk of CJD and vCJD in healthcare settings
***
Last updated 15 May 2015
***however in 1 C-type challenged animal, Prion 2015 Poster Abstracts S67
PrPsc was not detected using rapid tests for BSE.
***Subsequent testing resulted in the detection of pathologic lesion in
unusual brain location and PrPsc detection by PMCA only.
IBNC Tauopathy or TSE Prion disease, it appears, no one is sure
Posted by flounder on 03 Jul 2015 at 16:53 GMT
31 Jan 2015 at 20:14 GMT
*** Ruminant feed ban for cervids in the United States? ***
Singeltary et al
31 Jan 2015 at 20:14 GMT
Monday, October 26, 2015
FDA PART 589 -- SUBSTANCES PROHIBITED FROM USE IN ANIMAL FOOD OR FEED
VIOLATIONS OFFICIAL ACTION INDICATED OIA UPDATE October 2015
PL1
Using in vitro prion replication for high sensitive detection of prions and
prionlike proteins and for understanding mechanisms of transmission.
Claudio Soto
Mitchell Center for Alzheimer's diseases and related Brain disorders,
Department of Neurology, University of Texas Medical School at Houston.
Prion and prion-like proteins are misfolded protein aggregates with the
ability to selfpropagate to spread disease between cells, organs and in some
cases across individuals. I n T r a n s m i s s i b l e s p o n g i f o r m
encephalopathies (TSEs), prions are mostly composed by a misfolded form of the
prion protein (PrPSc), which propagates by transmitting its misfolding to the
normal prion protein (PrPC). The availability of a procedure to replicate prions
in the laboratory may be important to study the mechanism of prion and
prion-like spreading and to develop high sensitive detection of small quantities
of misfolded proteins in biological fluids, tissues and environmental samples.
Protein Misfolding Cyclic Amplification (PMCA) is a simple, fast and efficient
methodology to mimic prion replication in the test tube. PMCA is a platform
technology that may enable amplification of any prion-like misfolded protein
aggregating through a seeding/nucleation process. In TSEs, PMCA is able to
detect the equivalent of one single molecule of infectious PrPSc and propagate
prions that maintain high infectivity, strain properties and species
specificity. Using PMCA we have been able to detect PrPSc in blood and urine of
experimentally infected animals and humans affected by vCJD with high
sensitivity and specificity. Recently, we have expanded the principles of PMCA
to amplify amyloid-beta (Aβ) and alphasynuclein (α-syn) aggregates implicated in
Alzheimer's and Parkinson's diseases, respectively. Experiments are ongoing to
study the utility of this technology to detect Aβ and α-syn aggregates in
samples of CSF and blood from patients affected by these diseases.
=========================
***Recently, we have been using PMCA to study the role of environmental
prion contamination on the horizontal spreading of TSEs. These experiments have
focused on the study of the interaction of prions with plants and
environmentally relevant surfaces. Our results show that plants (both leaves and
roots) bind tightly to prions present in brain extracts and excreta (urine and
feces) and retain even small quantities of PrPSc for long periods of time.
Strikingly, ingestion of prioncontaminated leaves and roots produced disease
with a 100% attack rate and an incubation period not substantially longer than
feeding animals directly with scrapie brain homogenate. Furthermore, plants can
uptake prions from contaminated soil and transport them to different parts of
the plant tissue (stem and leaves). Similarly, prions bind tightly to a variety
of environmentally relevant surfaces, including stones, wood, metals, plastic,
glass, cement, etc. Prion contaminated surfaces efficiently transmit prion
disease when these materials were directly injected into the brain of animals
and strikingly when the contaminated surfaces were just placed in the animal
cage. These findings demonstrate that environmental materials can efficiently
bind infectious prions and act as carriers of infectivity, suggesting that they
may play an important role in the horizontal transmission of the disease.
========================
Since its invention 13 years ago, PMCA has helped to answer fundamental
questions of prion propagation and has broad applications in research areas
including the food industry, blood bank safety and human and veterinary disease
diagnosis.
see ;
98 | Veterinary Record | January 24, 2015
EDITORIAL
Scrapie: a particularly persistent pathogen
Cristina Acín
Resistant prions in the environment have been the sword of Damocles for
scrapie control and eradication. Attempts to establish which physical and
chemical agents could be applied to inactivate or moderate scrapie infectivity
were initiated in the 1960s and 1970s,with the first study of this type focusing
on the effect of heat treatment in reducing prion infectivity (Hunter and
Millson 1964). Nowadays, most of the chemical procedures that aim to inactivate
the prion protein are based on the method developed by Kimberlin and
collaborators (1983). This procedure consists of treatment with 20,000 parts per
million free chlorine solution, for a minimum of one hour, of all surfaces that
need to be sterilised (in laboratories, lambing pens, slaughterhouses, and so
on). Despite this, veterinarians and farmers may still ask a range of questions,
such as ‘Is there an official procedure published somewhere?’ and ‘Is there an
international organisation which recommends and defines the exact method of
scrapie decontamination that must be applied?’
From a European perspective, it is difficult to find a treatment that could
be applied, especially in relation to the disinfection of surfaces in lambing
pens of affected flocks. A 999/2001 EU regulation on controlling spongiform
encephalopathies (European Parliament and Council 2001) did not specify a
particular decontamination measure to be used when an outbreak of scrapie is
diagnosed. There is only a brief recommendation in Annex VII concerning the
control and eradication of transmissible spongiform encephalopathies (TSE s).
Chapter B of the regulation explains the measures that must be applied if
new caprine animals are to be introduced to a holding where a scrapie outbreak
has previously been diagnosed. In that case, the statement indicates that
caprine animals can be introduced ‘provided that a cleaning and disinfection of
all animal housing on the premises has been carried out following destocking’.
Issues around cleaning and disinfection are common in prion prevention
recommendations, but relevant authorities, veterinarians and farmers may have
difficulties in finding the specific protocol which applies. The European Food
and Safety Authority (EFSA ) published a detailed report about the efficacy of
certain biocides, such as sodium hydroxide, sodium hypochlorite, guanidine and
even a formulation of copper or iron metal ions in combination with hydrogen
peroxide, against prions (EFSA 2009). The report was based on scientific
evidence (Fichet and others 2004, Lemmer and others 2004, Gao and others 2006,
Solassol and others 2006) but unfortunately the decontamination measures were
not assessed under outbreak conditions.
The EFSA Panel on Biological Hazards recently published its conclusions on
the scrapie situation in the EU after 10 years of monitoring and control of the
disease in sheep and goats (EFSA 2014), and one of the most interesting findings
was the Icelandic experience regarding the effect of disinfection in scrapie
control. The Icelandic plan consisted of: culling scrapie-affected sheep or the
whole flock in newly diagnosed outbreaks; deep cleaning and disinfection of
stables, sheds, barns and equipment with high pressure washing followed by
cleaning with 500 parts per million of hypochlorite; drying and treatment with
300 ppm of iodophor; and restocking was not permitted for at least two years.
Even when all of these measures were implemented, scrapie recurred on several
farms, indicating that the infectious agent survived for years in the
environment, even as many as 16 years after restocking (Georgsson and others
2006).
In the rest of the countries considered in the EFSA (2014) report,
recommendations for disinfection measures were not specifically defined at the
government level. In the report, the only recommendation that is made for sheep
is repopulation with sheep with scrapie-resistant genotypes. This reduces the
risk of scrapie recurrence but it is difficult to know its effect on the
infection.
Until the EFSA was established (in May 2003), scientific opinions about TSE
s were provided by the Scientific Steering Committee (SSC) of the EC, whose
advice regarding inactivation procedures focused on treating animal waste at
high temperatures (150°C for three hours) and high pressure alkaline hydrolysis
(SSC 2003). At the same time, the TSE Risk Management Subgroup of the Advisory
Committee on Dangerous Pathogens (ACDP) in the UK published guidance on safe
working and the prevention of TSE infection. Annex C of the ACDP report
established that sodium hypochlorite was considered to be effective, but only if
20,000 ppm of available chlorine was present for at least one hour, which has
practical limitations such as the release of chlorine gas, corrosion,
incompatibility with formaldehyde, alcohols and acids, rapid inactivation of its
active chemicals and the stability of dilutions (ACDP 2009).
In an international context, the World Organisation for Animal Health (OIE)
does not recommend a specific disinfection protocol for prion agents in its
Terrestrial Code or Manual. Chapter 4.13 of the Terrestrial Code, General
recommendations on disinfection and disinsection (OIE 2014), focuses on
foot-and-mouth disease virus, mycobacteria and Bacillus anthracis, but not on
prion disinfection. Nevertheless, the last update published by the OIE on bovine
spongiform encephalopathy (OIE 2012) indicates that few effective
decontamination techniques are available to inactivate the agent on surfaces,
and recommends the removal of all organic material and the use of sodium
hydroxide, or a sodium hypochlorite solution containing 2 per cent available
chlorine, for more than one hour at 20ºC.
The World Health Organization outlines guidelines for the control of TSE s,
and also emphasises the importance of mechanically cleaning surfaces before
disinfection with sodium hydroxide or sodium hypochlorite for one hour (WHO
1999).
Finally, the relevant agencies in both Canada and the USA suggest that the
best treatments for surfaces potentially contaminated with prions are sodium
hydroxide or sodium hypochlorite at 20,000 ppm. This is a 2 per cent solution,
while most commercial household bleaches contain 5.25 per cent sodium
hypochlorite. It is therefore recommended to dilute one part 5.25 per cent
bleach with 1.5 parts water (CDC 2009, Canadian Food Inspection Agency 2013).
So what should we do about disinfection against prions? First, it is
suggested that a single protocol be created by international authorities to
homogenise inactivation procedures and enable their application in all
scrapie-affected countries. Sodium hypochlorite with 20,000 ppm of available
chlorine seems to be the procedure used in most countries, as noted in a paper
summarised on p 99 of this issue of Veterinary Record (Hawkins and others 2015).
But are we totally sure of its effectiveness as a preventive measure in a
scrapie outbreak? Would an in-depth study of the recurrence of scrapie disease
be needed?
What we can conclude is that, if we want to fight prion diseases, and
specifically classical scrapie, we must focus on the accuracy of diagnosis,
monitoring and surveillance; appropriate animal identification and control of
movements; and, in the end, have homogeneous and suitable protocols to
decontaminate and disinfect lambing barns, sheds and equipment available to
veterinarians and farmers. Finally, further investigations into the resistance
of prion proteins in the diversity of environmental surfaces are required.
References
snip...
98 | Veterinary Record | January 24, 2015
Persistence of ovine scrapie infectivity in a farm environment following
cleaning and decontamination
Steve A. C. Hawkins, MIBiol, Pathology Department1, Hugh A. Simmons, BVSc
MRCVS, MBA, MA Animal Services Unit1, Kevin C. Gough, BSc, PhD2 and Ben C.
Maddison, BSc, PhD3 + Author Affiliations
1Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey
KT15 3NB, UK 2School of Veterinary Medicine and Science, The University of
Nottingham, Sutton Bonington, Loughborough, Leicestershire LE12 5RD, UK 3ADAS
UK, School of Veterinary Medicine and Science, The University of Nottingham,
Sutton Bonington, Loughborough, Leicestershire LE12 5RD, UK E-mail for
correspondence: ben.maddison@adas.co.uk Abstract Scrapie of sheep/goats and
chronic wasting disease of deer/elk are contagious prion diseases where
environmental reservoirs are directly implicated in the transmission of disease.
In this study, the effectiveness of recommended scrapie farm decontamination
regimens was evaluated by a sheep bioassay using buildings naturally
contaminated with scrapie. Pens within a farm building were treated with either
20,000 parts per million free chorine solution for one hour or were treated with
the same but were followed by painting and full re-galvanisation or replacement
of metalwork within the pen. Scrapie susceptible lambs of the PRNP genotype
VRQ/VRQ were reared within these pens and their scrapie status was monitored by
recto-anal mucosa-associated lymphoid tissue. All animals became infected over
an 18-month period, even in the pen that had been subject to the most stringent
decontamination process. These data suggest that recommended current guidelines
for the decontamination of farm buildings following outbreaks of scrapie do
little to reduce the titre of infectious scrapie material and that environmental
recontamination could also be an issue associated with these premises.
SNIP...
Discussion
Thorough pressure washing of a pen had no effect on the amount of
bioavailable scrapie infectivity (pen B). The routine removal of prions from
surfaces within a laboratory setting is treatment for a minimum of one hour with
20,000 ppm free chlorine, a method originally based on the use of brain
macerates from infected rodents to evaluate the effectiveness of decontamination
(Kimberlin and others 1983). Further studies have also investigated the
effectiveness of hypochlorite disinfection of metal surfaces to simulate the
decontamination of surgical devices within a hospital setting. Such treatments
with hypochlorite solution were able to reduce infectivity by 5.5 logs to lower
than the sensitivity of the bioassay used (Lemmer and others 2004). Analogous
treatment of the pen surfaces did not effectively remove the levels of scrapie
infectivity over that of the control pens, indicating that this method of
decontamination is not effective within a farm setting. This may be due to the
high level of biological matrix that is present upon surfaces within the farm
environment, which may reduce the amount of free chlorine available to
inactivate any infectious prion. Remarkably 1/5 sheep introduced into pen D had
also became scrapie positive within nine months, with all animals in this pen
being RAMALT positive by 18 months of age. Pen D was no further away from the
control pen (pen A) than any of the other pens within this barn. Localised hot
spots of infectivity may be present within scrapie-contaminated environments,
but it is unlikely that pen D area had an amount of scrapie contamination that
was significantly different than the other areas within this building.
Similarly, there were no differences in how the biosecurity of pen D was
maintained, or how this pen was ventilated compared with the other pens. This
observation, perhaps, indicates the slower kinetics of disease uptake within
this pen and is consistent with a more thorough prion removal and
recontamination. These observations may also account for the presence of
inadvertent scrapie cases within other studies, where despite stringent
biosecurity, control animals have become scrapie positive during challenge
studies using barns that also housed scrapie-affected animals (Ryder and others
2009).
***The bioassay data indicate that the exposure of the sheep to a farm
environment after decontamination efforts thought to be effective in removing
scrapie is sufficient for the animals to become infected with scrapie. The main
exposure routes within this scenario are likely to be via the oral route, during
feeding and drinking, and respiratory and conjunctival routes. It has been
demonstrated that scrapie infectivity can be efficiently transmitted via the
nasal route in sheep (Hamir and others 2008), as is the case for CWD in both
murine models and in white-tailed deer (Denkers and others 2010, 2013).
Recently, it has also been demonstrated that CWD prions presented as dust
when bound to the soil mineral montmorillonite can be infectious via the nasal
route (Nichols and others 2013). When considering pens C and D, the actual
source of the infectious agent in the pens is not known, it is possible that
biologically relevant levels of prion survive on surfaces during the
decontamination regimen (pen C). With the use of galvanising and painting (pen
D) covering and sealing the surface of the pen, it is possible that scrapie
material recontaminated the pens by the movement of infectious prions contained
within dusts originating from other parts of the barn that were not
decontaminated or from other areas of the farm.
Given that scrapie prions are widespread on the surfaces of affected farms
(Maddison and others 2010a), irrespective of the source of the infectious prions
in the pens, this study clearly highlights the difficulties that are faced with
the effective removal of environmentally associated scrapie infectivity. This is
likely to be paralleled in CWD which shows strong similarities to scrapie in
terms of both the dissemination of prions into the environment and the facile
mode of disease transmission. These data further contribute to the understanding
that prion diseases can be highly transmissible between susceptible individuals
not just by direct contact but through highly stable environmental reservoirs
that are refractory to decontamination.
The presence of these environmentally associated prions in farm buildings
make the control of these diseases a considerable challenge, especially in
animal species such as goats where there is lack of genetic resistance to
scrapie and, therefore, no scope to re-stock farms with animals that are
resistant to scrapie.
Scrapie Sheep Goats Transmissible spongiform encephalopathies (TSE)
Accepted October 12, 2014. Published Online First 31 October 2014
Monday, November 3, 2014
Persistence of ovine scrapie infectivity in a farm environment following
cleaning and decontamination
PPo3-22:
Detection of Environmentally Associated PrPSc on a Farm with Endemic
Scrapie
Ben C. Maddison,1 Claire A. Baker,1 Helen C. Rees,1 Linda A. Terry,2 Leigh
Thorne,2 Susan J. Belworthy2 and Kevin C. Gough3 1ADAS-UK LTD; Department of
Biology; University of Leicester; Leicester, UK; 2Veterinary Laboratories
Agency; Surry, KT UK; 3Department of Veterinary Medicine and Science; University
of Nottingham; Sutton Bonington, Loughborough UK
Key words: scrapie, evironmental persistence, sPMCA
Ovine scrapie shows considerable horizontal transmission, yet the routes of
transmission and specifically the role of fomites in transmission remain poorly
defined. Here we present biochemical data demonstrating that on a
scrapie-affected sheep farm, scrapie prion contamination is widespread. It was
anticipated at the outset that if prions contaminate the environment that they
would be there at extremely low levels, as such the most sensitive method
available for the detection of PrPSc, serial Protein Misfolding Cyclic
Amplification (sPMCA), was used in this study. We investigated the distribution
of environmental scrapie prions by applying ovine sPMCA to samples taken from a
range of surfaces that were accessible to animals and could be collected by use
of a wetted foam swab. Prion was amplified by sPMCA from a number of these
environmental swab samples including those taken from metal, plastic and wooden
surfaces, both in the indoor and outdoor environment. At the time of sampling
there had been no sheep contact with these areas for at least 20 days prior to
sampling indicating that prions persist for at least this duration in the
environment. These data implicate inanimate objects as environmental reservoirs
of prion infectivity which are likely to contribute to disease transmission.
the tse prion aka mad cow type disease is not your normal pathogen.
The TSE prion disease survives ashing to 600 degrees celsius, that’s around
1112 degrees farenheit.
you cannot cook the TSE prion disease out of meat. you can take the ash and
mix it with saline and inject that ash into a mouse, and the mouse will go down
with TSE.
Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel
Production as well.
the TSE prion agent also survives Simulated Wastewater Treatment Processes.
IN fact, you should also know that the TSE Prion agent will survive in the
environment for years, if not decades.
you can bury it and it will not go away.
The TSE agent is capable of infected your water table i.e. Detection of
protease-resistant cervid prion protein in water from a CWD-endemic area.
it’s not your ordinary pathogen you can just cook it out and be done with.
that’s what’s so worrisome about Iatrogenic mode of transmission, a simple
autoclave will not kill this TSE prion agent.
cwd to humans, consumption, exposure, sub-clinical, iatrogenic, what if ?
Saturday, January 31, 2015
European red deer (Cervus elaphus elaphus) are susceptible to Bovine
Spongiform Encephalopathy BSE by Oral Alimentary route
*** Singeltary reply ; Molecular, Biochemical and Genetic Characteristics
of BSE in Canada Singeltary reply ;
Saturday, March 21, 2015
*** Canada and United States Creutzfeldt Jakob TSE Prion Disease Incidence
Rates Increasing
*** O.05: Transmission of prions to primates after extended silent
incubation periods: ***Implications for BSE and scrapie risk assessment in human
populations
Emmanuel Comoy, Jacqueline Mikol, Valerie Durand, Sophie Luccantoni,
Evelyne Correia, Nathalie Lescoutra, Capucine Dehen, and Jean-Philippe Deslys
Atomic Energy Commission; Fontenay-aux-Roses, France
Prion diseases (PD) are the unique neurodegenerative proteinopathies
reputed to be transmissible under field conditions since decades. The
transmission of Bovine Spongiform Encephalopathy (BSE) to humans evidenced that
an animal PD might be zoonotic under appropriate conditions. Contrarily, in the
absence of obvious (epidemiological or experimental) elements supporting a
transmission or genetic predispositions, PD, like the other proteinopathies, are
reputed to occur spontaneously (atpical animal prion strains, sporadic CJD
summing 80% of human prion cases). Non-human primate models provided the first
evidences supporting the transmissibiity of human prion strains and the zoonotic
potential of BSE. Among them, cynomolgus macaques brought major information for
BSE risk assessment for human health (Chen, 2014), according to their
phylogenetic proximity to humans and extended lifetime. We used this model to
assess the zoonotic potential of other animal PD from bovine, ovine and cervid
origins even after very long silent incubation periods.
*** We recently observed the direct transmission of a natural classical
scrapie isolate to macaque after a 10-year silent incubation period,
***with features similar to some reported for human cases of sporadic CJD,
albeit requiring fourfold longe incubation than BSE. Scrapie, as recently evoked
in humanized mice (Cassard, 2014),
***is the third potentially zoonotic PD (with BSE and L-type BSE),
***thus questioning the origin of human sporadic cases. We will present an
updated panorama of our different transmission studies and discuss the
implications of such extended incubation periods on risk assessment of animal PD
for human health.
===============
***thus questioning the origin of human sporadic cases...TSS
===============
2014
***Moreover, L-BSE has been transmitted more easily to transgenic mice
overexpressing a human PrP [13,14] or to primates [15,16] than C-BSE.
***It has been suggested that some sporadic CJD subtypes in humans may
result from an exposure to the L-BSE agent.
*** Lending support to this hypothesis, pathological and biochemical
similarities have been observed between L-BSE and an sCJD subtype (MV genotype
at codon 129 of PRNP) [17], and between L-BSE infected non-human primate and
another sCJD subtype (MM genotype) [15].
snip...
PRION 2015 CONFERENCE FT. COLLINS CWD RISK FACTORS TO HUMANS
*** LATE-BREAKING ABSTRACTS PRION 2015 CONFERENCE ***
O18
Zoonotic Potential of CWD Prions
Liuting Qing1, Ignazio Cali1,2, Jue Yuan1, Shenghai Huang3, Diane Kofskey1,
Pierluigi Gambetti1, Wenquan Zou1, Qingzhong Kong1 1Case Western Reserve
University, Cleveland, Ohio, USA, 2Second University of Naples, Naples, Italy,
3Encore Health Resources, Houston, Texas, USA
*** These results indicate that the CWD prion has the potential to infect
human CNS and peripheral lymphoid tissues and that there might be asymptomatic
human carriers of CWD infection.
==================
***These results indicate that the CWD prion has the potential to infect
human CNS and peripheral lymphoid tissues and that there might be asymptomatic
human carriers of CWD infection.***
==================
P.105: RT-QuIC models trans-species prion transmission
Kristen Davenport, Davin Henderson, Candace Mathiason, and Edward Hoover
Prion Research Center; Colorado State University; Fort Collins, CO USA
Conversely, FSE maintained sufficient BSE characteristics to more
efficiently convert bovine rPrP than feline rPrP. Additionally, human rPrP was
competent for conversion by CWD and fCWD.
***This insinuates that, at the level of protein:protein interactions, the
barrier preventing transmission of CWD to humans is less robust than previously
estimated.
================
***This insinuates that, at the level of protein:protein interactions, the
barrier preventing transmission of CWD to humans is less robust than previously
estimated.***
================
From: Terry S. Singeltary Sr.
Sent: Saturday, November 15, 2014 9:29 PM
To: Terry S. Singeltary Sr.
Subject: THE EPIDEMIOLOGY OF CREUTZFELDT-JAKOB DISEASE R. G. WILL 1984
THE EPIDEMIOLOGY OF CREUTZFELDT-JAKOB DISEASE
R. G. WILL
1984
*** The association between venison eating and risk of CJD shows similar
pattern, with regular venison eating associated with a 9 FOLD INCREASE IN RISK
OF CJD (p = 0.04). (SEE LINK IN REPORT HERE...TSS) PLUS, THE CDC DID NOT PUT
THIS WARNING OUT FOR THE WELL BEING OF THE DEER AND ELK ;
snip...
*** These results would seem to suggest that CWD does indeed have zoonotic
potential, at least as judged by the compatibility of CWD prions and their human
PrPC target. Furthermore, extrapolation from this simple in vitro assay suggests
that if zoonotic CWD occurred, it would most likely effect those of the PRNP
codon 129-MM genotype and that the PrPres type would be similar to that found in
the most common subtype of sCJD (MM1).***
*** The potential impact of prion diseases on human health was greatly
magnified by the recognition that interspecies transfer of BSE to humans by beef
ingestion resulted in vCJD. While changes in animal feed constituents and
slaughter practices appear to have curtailed vCJD, there is concern that CWD of
free-ranging deer and elk in the U.S. might also cross the species barrier.
Thus, consuming venison could be a source of human prion disease. Whether BSE
and CWD represent interspecies scrapie transfer or are newly arisen prion
diseases is unknown. Therefore, the possibility of transmission of prion disease
through other food animals cannot be ruled out. There is evidence that vCJD can
be transmitted through blood transfusion. There is likely a pool of unknown size
of asymptomatic individuals infected with vCJD, and there may be asymptomatic
individuals infected with the CWD equivalent. These circumstances represent a
potential threat to blood, blood products, and plasma supplies. ***
PRION CONFERENCE 2014 HELD IN ITALY RECENTLY CWD BSE TSE UPDATE
> First transmission of CWD to transgenic mice over-expressing bovine
prion protein gene (TgSB3985)
PRION 2014 - PRIONS: EPIGENETICS and NEURODEGENERATIVE DISEASES – Shaping
up the future of prion research
Animal TSE Workshop 10.40 – 11.05 Talk Dr. L. Cervenakova First
transmission of CWD to transgenic mice over-expressing bovine prion protein gene
(TgSB3985)
Friday, August 14, 2015
*** Susceptibility of cattle to the agent of chronic wasting disease from
elk after intracranial inoculation
July's Milwaukee Journal Sentinel article did prod state officials to ask
CDC to investigate the cases of the three men who shared wild game feasts. The
two men the CDC is still investigating were 55 and 66 years old. But there's
also Kevin Boss, a Minnesota hunter who ate Barron County venison and died of
CJD at 41. And there's Jeff Schwan, whose Michigan Tech fraternity brothers used
to bring venison sausage back to the frat house. His mother, Terry, says that in
May 2001, Jeff, 26, began complaining about his vision. A friend noticed
misspellings in his e-mail, which was totally unlike him. Jeff began losing
weight. He became irritable and withdrawn. By the end of June, he couldn't
remember the four-digit code to open the garage door or when and how to feed his
parents' cats. At a family gathering in July, he stuck to his parents and
girlfriend, barely talking. "On the night we took him to the hospital, he was
speaking like he was drunk or high and I noticed his pupils were so dilated I
couldn't see the irises," his mother says. By then, Jeff was no longer able to
do even simple things on his computer at work, and "in the hospital, he couldn't
drink enough water." When he died on September 27, 2001, an autopsy confirmed he
had sporadic CJD.
In 2000, Belay looked into three CJD cases reported by The Denver Post, two
hunters who ate meat from animals killed in Wyoming and the daughter of a hunter
who ate venison from a plant that processed Colorado elk. All three died of CJD
before they were 30 years old. The CDC asked the USDA to kill 1,000 deer and elk
in the area where the men hunted. Belay and others reported their findings in
the Archives of Neurology, writing that although "circumstances suggested a link
between the three cases and chronic wasting disease, they could find no 'causal'
link." Which means, says Belay, "not a single one of those 1,000 deer tested
positive for CWD. For all we know, these cases may be CWD. What we have now
doesn't indicate a connection. That's reassuring, but it would be wrong to say
it will never happen."
So far, says NIH researcher Race, the two Wisconsin cases pinpointed by the
newspaper look like spontaneous CJD. "But we don't know how CWD would look in
human brains. It probably would look like some garden-variety sporadic CJD."
What the CDC will do with these cases and four others (three from Colorado and
Schwan from Upper Michigan), Race says, is "sequence the prion protein from
these people, inject it into mice and wait to see what the disease looks like in
their brains. That will take two years."
CJD is so rare in people under age 30, one case in a billion (leaving out
medical mishaps), that four cases under 30 is "very high," says Colorado
neurologist Bosque. "Then, if you add these other two from Wisconsin [cases in
the newspaper], six cases of CJD in people associated with venison is very, very
high." Only now, with Mary Riley, there are at least seven, and possibly eight,
with Steve, her dining companion. "It's not critical mass that matters,"
however, Belay says. "One case would do it for me." The chance that two people
who know each other would both contact CJD, like the two Wisconsin sportsmen, is
so unlikely, experts say, it would happen only once in 140 years.
Given the incubation period for TSEs in humans, it may require another
generation to write the final chapter on CWD in Wisconsin. "Does chronic wasting
disease pass into humans? We'll be able to answer that in 2022," says Race.
Meanwhile, the state has become part of an immense experiment.
I urge everyone to watch this video closely...terry
*** you can see video here and interview with Jeff's Mom, and scientist
telling you to test everything and potential risk factors for humans ***
Sunday, October 25, 2015
USAHA Detailed Events Schedule – 119th USAHA Annual Meeting CAPTIVE
LIVESTOCK CWD SCRAPIE TSE PRION
Sunday, October 18, 2015
World Organisation for Animal Health (OIE) and the Institut Pasteur
Cooperating on animal disease and zoonosis research
31 Jan 2015 at 20:14 GMT
*** Ruminant feed ban for cervids in the United States? ***
Singeltary et al
31 Jan 2015 at 20:14 GMT
Monday, October 26, 2015
FDA PART 589 -- SUBSTANCES PROHIBITED FROM USE IN ANIMAL FOOD OR FEED
VIOLATIONS OFFICIAL ACTION INDICATED OIA UPDATE October 2015
Mad deer disease can infect normal human brains in laboratory tests
Monday, May 8, 2000
www.ems.org has further information and facts on CJD. Jennifer Kelly or Amy
Leska, EMS 202/463-6670 Washington, D.C.
Public health advocates are demanding that the Food and Drug Administration
close loopholes in animal feed regulations to prevent the spread of U.S. mad
cow-type diseases now at epidemic levels in Western deer and elk that might
infect people who eat meat. In a letter sent today to the FDA, the Center for
Food Safety (CFS), the Humane Farming Association, and families of U.S. victims
of the human version of mad cow disease, Creutzfeldt-Jakob disease (CJD) are
demanding new efforts to protect public health and food safety. The FDA was
asked to respond to a legal petition filed in January 1999 that would change
U.S. animal feed regulations to prevent the spread of U.S. mad cow-type diseases
already occurring in deer, elk, sheep and humans, and suspected in pigs and
cattle.
Under current FDA regulations animals known to be infected with mad
cow-type disease such as deer and elk infected with 'chronic wasting disease'
and scrapie-infected sheep, can be legally fed to pigs, chickens and pets, which
in turn can be rendered and fed to cows. Billions of pounds of slaughterhouse
waste in the form of rendered animal by-products are fed to US livestock every
year as fat and protein supplements, despite this practice being the known route
of transmission of British mad cow disease.
A fatal 'mad deer' disease called chronic wasting disease or CWD is
occurring at epidemic levels in deer and elk in Western states and on game
farms, CFS legal director Joseph Mendelson wrote in the letter to the FDA. This
may already be claiming human lives as is suggested by the alarming appearance
of unusually young victims of Creutzfeldt-Jakob disease.
*** Today at the first CJD Foundation conference in Miami, government
researcher Byron W. Caughey, Ph.D., announced that in laboratory tests CWD from
deer can infect human brain tissue at rate similar to British mad cow disease.
In Britain 56 people have died of human mad cow disease, the death toll is
climbing and some scientists suspect it will claim hundreds of thousands of
lives in the decades ahead. Caughey's research on US mad deer disese was
conducted at the National Institutes of Health Rocky Mountain Laboratories in
Hamilton, Montana, and has not yet been published.
The most recent suspected victim of US mad deer disease is Jay Dee Whitlock
II of Oklahoma, who died of CJD on April 7, 2000. Whitlock, 28, was an avid deer
hunter and venison consumer. He is the second young hunter to die of CJD in the
past year.
John Stauber, co-author of Mad Cow USA and a speaker at the CJD Foundation
conference, said, "The announcement that US mad deer disease can infect the
human brain, and that it happens at a rate similar to British mad cow disease,
is extremely disturbing. A deadly human dementia might be already spreading from
deer and elk into hunters in Western states, and the policies of the FDA and
other agencies are completely inadequate to protect public health.
Could 'mad cow type epidemics happen in the US John Stauber, talk given 7
May 00 CJD Foundation conference Miami Center for Media & Democracy 520
University Avenue, Suite #310 Madison, WI 53703
"Three years ago Sheldon Rampton and I were finishing our book Mad Cow USA
published in November of 97. In our introduction we wrote that ours is not a
biology book nor a diet guide, but a "book about politics and how it operates in
the real world. It explains how government officials have placed concerns for
the food industry over human health and welfare. In addition to telling the
story of an exotic, mysterious and frightening disease, we have written this
book to report on equally dangerous legal and political trends which threaten
not only our physical health, but also our fundamental democratic rights..." The
title of this session asks a question: "Could 'mad cow type epidemics happen in
the US.?" My answer is not only could they, but they are. Sheep scrapie arrived
in the US a half century ago and thanks to government bungling is now widely
spread throughout the US, with dozens of different strains. Chronic wasting
disease may have begun as sheep scrapie but now it is spreading through deer and
elk in western states and on game farms.
In the past two years two western state hunters under the age of 30 have
died of CJD, and some of us suspect they may be human victims of a new strain of
TSE in sheep, deer or elk that has begun claiming young human victims in the US.
Since 1964 researchers have suspected that transmissible mink
encephalopathy TME in the US is from a hidden TSE in cattle, and the late Dr.
Richard Marsh, to whom we dedicate our book, believed that he isolated that TSE
in mink in Wisconsin in 1985, a year before the British strain of TSE dubbed mad
cow disease was observed.
As our book was going to completion, we discovered that USDA inspectors
felt they had identified a TSE in pigs way back in the the 1970s, when few
researchers other than a small group in what Paul Brown calls the Club were very
concerned or aware of TSE diseases.
Of course, British mad cow disease and the looming specter of hundreds of
thousands of people condemned to death in the decades ahead has changed
everything. Everything, that is, except public policy here in the US. For
despite the fact that two Nobel prize winners in this area of research are from
the US, there is a public relations cover-up of massive proportions in this
country that is preventing us from effectively establishing public policies that
can monitor, prevent and eventually treat TSE diseases.
Please note that I said a public relations cover-up; that's important. I
suspect that if TSE diseases were spread by mosquitoes, we'd be spraying
pesticides all over the US to try and irradiate sheep scrapie and chronic
wasting disease. But these diseases are spread by agribusiness through animal
livestock products fed to people and animals, and thus instead of putting the
concerns of people first, we have see that consistently governments have put the
concerns of industry first.
We've heard eminent and hard working scientists talk about TSE diseases in
terms that dizzy the head of even other scientists. This is a very contentious
and mysterious area of research because TSE diseases break many rules.
Luckily, my co-author and I are not experts or scientists. However, we did
have the benefit of being able to interview top TSE researchers like Richard
Marsh and Dr. Gajdusek, have them review parts of our manuscript, and thus
deliver a book that is based on solid, sound science regarding what is known,
and what is unknown, what is proven, and what is not.
From my perspective, this is the most important point and perspective to
keep in mind:
For the past thirty years a massive unregulated experiment in creating new
strains of TSE disease has been undertaken by the livestock industry, and we're
the guinea pigs. The experiment is ongoing. The experiment began as a really
neat idea: lets take the billions and billions of pounds of slaughterhouse
waste, blood and offal that is produced every year from cattle and pigs and
sheep and roadkill and pets and zoo animals, and let's recycle it. Let's turn it
into protein fat supplements, and let's feed it back to the livestock we eat.
It seemed like a good idea. Unfortunately, what it didn't take into account
was the infectious prion agent. As Dr. Gibbs has pointed out, probably every
mammalian species has a TSE disease at some minute level: people, pigs, cows,
sheep, mink, cats and dogs, deer, elk. Grind up the most infectious parts of
millions and millions of animals, concentrate them into feed supplements, and
you are creating an environment not only for spreading and amplifying existing
agents such as scrapie in sheep, but also for creating an untold number of new
strains of TSE.
This is especially important to grasp. In laboratory experiments, when TME
or scrapie or CJD is injected into new animal species, whole new strains of TSE
are created, and they can have different potential to infect new host species.
The process of rendering animal waste into animal feed has been a massive and
ongoing experiment in the creation of new TSEs, one of which emerged in Britain
in the mid 1980s as mad cow disease, has clearly spread into humans claiming
over fifty so far, and in my guess will in the years ahead be founded to have
infected hundreds of thousands of Britons.
I said that this outbreak of BSE in Britain changed everything, but what
really changed everything was what caused that outbreak: feeding rendered animal
by-products back to animals as food.
I wish I could tell you that this practice has been stopped, but it has
not. I wish I could tell you that well informed and courageous officials in the
FDA, the USDA and the CDC have taken the mad bull by the horns, and are right
now executing a precautionary policy to insure that what has happened in Britain
does not happen in the US. Unfortunately, that is not the case.
Dr. Hansen will shortly explain why the much heralded FDA feed regulations
on rendered animal by-products announced in 1997 are in many ways a farce. For
instance, in the US calves are literally being weaned on cattle blood protein,
and scrapie infected sheep can be used as feed for pigs which can be used as
feed for cattle.
But the problem is beyond just the poor US animal feed regulations. You
need look no further than your favorite vitamin and nutritional supplement
stores. Right now, with the full knowledge of the FDA, the NIH, the CDC,
millions of Americans are popping over the counter as glandular supplements.
These unregulated products contain the most infectious parts of slaughtered
animals, the very parts that make up the so-called Specified Bovine Offal that
should be banned from cattle feed, such as the brain, pituitary, and glandular
system. These pills are pooled, collected from hundreds of thousands of animals
and taken daily by untold numbers of Americans, probably millions given the
popularity of supplements.
Again, this amounts to an unregulated human experiment, minus laboratory
controls or knowledgeable consent, feeding humans the most infectious parts of
animals, possible creating new TSEs by passaging animal TSE from pooled
glandular products.
I've brought along four different bottles of the pills I'm talking about,
and I've asked that they be pass around so you can look at them yourself and
read the label.
Some would say that FDA's hands are tied, that thanks to lobbying by the
nutritional supplement industry the FDA lacks the power to prevent sales of
these glandular. Actually this points to another frightening disease rampant
among otherwise good people who populate government agencies which I call BCD
for Bureaucratic Cowardice Disease. FDAs hands might be tied and they can't yank
these products from the market, but their mouths are not gagged and concerned
officials should be speaking out loudly warning the American public that they
are consuming animal brains and glands and might want to consider the potential
risks.
There is a reason why scientists in agencies and universities, not to
mention corporations, avoid sticking their necks out, and we need look no
further than Richard Marsh, a dedicated and conservative scientist who took it
upon himself to speak up in 1993 in an interview in Wisconsin's largest farm
paper. Richard Marsh warned Wisconsin farmers, thousands of whom were feeding
rendered cow by-products back to their cows, that given the mad cow outbreak in
Britain they should, despite the reassurances from USDA that such feeding was
safe, stop.
The day after that article appeared Dr. Marsh was literally called on the
carpet in the office of the dean of the school of agriculture at the University
of Wisconsin, and read the riot act. he was told that industry funders were
threaten to sue him, sue the school, cut off money for research, and who did he
think he was?
Within months the school scrambled and pulled together a symposium on the
subject, which served to dismiss and marginalize Dr. Marsh's views. When Dr.
Marsh died of cancer in 1997, after the British announcement that mad cow
disease was killing young people, the same University had the gall to praise Dr.
Marsh and his work as in the best tradition of the University. Some of us feel
that the stress and abuse heaped on Dr. Marsh> from 1993 to 1997 had an
impact on his health that contributed to his demise.
This is what happens to scientists who break ranks and speak out, and it is
a great loss to us today because I think if Dick Marsh was still alive he would
be leading the charge to confront the ignorance and the cow-towing to industry
that typifies this issue.
Dr. Marsh was a colleague and contemporary of many of the researchers in
this room. As Paul Brown whom he once worked with might say, he was a member of
the club. But Dr. Marsh was able to make realizations that I'm afraid most of
the club members haven't yet come to, and he was able to put his sense of
scientific obligation to society in front of concerns about his funding, or even
his personal and professional safety.
I first began investigating mad cow type diseases in the early 1990s when I
was working in Wisconsin organizing farmers and consumers opposed to Monsanto's
genetically engineered bovine growth hormone, the cattle equivalent of human
growth hormone which when injected into cattle forced them to produce more milk.
Well, its not quite that biologically simple as I'm sure any woman here who has
had children can imagine.
It was brought to my attention by a retired industry veterinary researcher
that in order to make the hormone work, cows need to be fed additional fat and
protein supplements, and that the cheapest supplements were rendered byproducts
from other cows. This veterinarian told me this was very bad because it was
exactly what had cause the outbreak of BSE in Britain.
I investigated and found this was true - massive feeding of cows to cows
was going on in the US, despite the fact that as early as the Fall of 1987
British epidemiology had shown that is was this practice that spread mad cow
disease.
I remember one of my first meetings with Dr. Marsh. We talked about his
believe that a US BSE agent, different than the British strain, was in cattle at
low levels and was the cause of occasional outbreaks of TME. I know that eminent
researchers in this room dispute that, but frankly Dr. Marsh never doubted it
and I think the evidence and commons sense remains in his favor.
I had just been to a holistic chiropractor for my chronically bad lower
back, and had been sold a bottle of adrenal gland extract which I showed to Dr.
Marsh - he practically fell out of his chair and I immediately stopped taking
them.
He was shocked to learn that such glandular were sold. Imagine what he
might think today, that at this late stage of the game with kids in their 20s in
the US dying of CJD, that these glandulars are sold without warning.
Of course, I may be taking a risk myself in saying this. After all, we are
in Florida, one of 13 states in the US in which the Animal Feed Industry
Association members have succeeded in lobbying into law a food product
disparagement bill. I could end up like Oprah Winfrey and her guest Howard
Lyman, forced to spend millions and millions of dollars (which in my case would
be thousands and thousands and then bankruptcy) to convince a jury that my
remarks today are based on sound science.
Having written two books in the past five years, one on political PR and
the other on the politics of food, I can tell you that a major reason why there
has not been more news media coverage of this issue is the multi-million dollar
PR campaign and litigation threats of the food industry which we document
extensively in our book.
I find it ironic that this weekend as we're meeting here President Clinton
announced Saturday a new initiative to address the safety of hot dogs. Philip
Brasher of the Associated Press speculates that as he is leaving office the
President wants to burnish his image with food safety initiatives.
The irony is that it is under this administration that the food industry
has launched an all out attack on our first amendment rights by lobbying food
libel laws onto the books, and the administration has remained silent. Despite
knowing of the risks of cow cannibalism since the beginning of his presidency,
his administration did nothing until 1997, and as we reveal in our book and Dr.
Hansen will explain, the regulations are severely inadequate.
I am circulating a letter being delivered by the Center for Food Safety
urging the agency to respond to the petition filed by that group and many of the
families in this room that calls on FDA to severely tighten its regulations.
Yet, probably to avoid publicity, the FDA stonewalls.
A couple weeks ago I was in Washington and I attended a conference that was
paid for in part by the lobbyists responsible for putting food censorships laws
on the books, the Animal Feed Industry Association and the law firm of Ollsen
Frank and Weeda that drafted the model bill that was then lobbied for at the
state level by the American Farm Bureau. I picked up this glossy brochure in
which the FDA is slapping itself on the back for its food safety initiatives. In
it I read that the FDA's BSE Educational Activities have Gained an award from
the vice president:
The US feed regulations and the FDA were honored with VP Al Gore's Hammer
Award. Dr. Stephen F. Sundlof, the directory of FDA's Center for Veterinary
Medicine, the man who since January 1999 has been unable to respond to the legal
petition to close gaping loopholes in the FDA regulations stated: "thanks to the
development of the BSE regulation, we can continue to say that there has not
been a single case of BSE reported in the United States. Educational efforts ...
will help assure that we can continue to make that claim."
In researching our books we had access to documents obtained through the
Freedom on Information Act. One 1991 document of the USDA was titled "BSE Public
Relations," and if someday attorneys in a class action lawsuit on behalf of CJD
patients in the US are looking for a smoking gun demonstrating that concerns for
industry were place over concerns for people, this is it.
(p.148-149) According to this document, the mere perception that BSE might
exist in the United States could have devastating effects on our domestic
markets for beef and dairy products." The report examined how the British
handled their PR, and it fretted over a story in the British magazine The
Economist which, quote, "could potentially create alarm among US consumers,"
unquote, because it reported that, quote,"many veterinarians and medical experts
have come around to the belief that humans could catch the mystery brain
disease."
This USDA PR document approvingly noted a quote in the Washington Post by
Dr. Joseph Gibbs at NIH in 1990 saying "I don't think there is any danger in
consuming British beef." Remember, this was in 1991. It had known for four years
that it was feeding cows to cows that was spreading BSE in Britain. More and
more were fearing that humans would die. Meanwhile, in the US, billions of
pounds of cow by-products were still being fed, in fact the amount was
increasing each year.
Was there no one inside the USDA or FDA who saw the lunacy, indeed the
criminality, in knowing this and letting cannibalistic feeding go one in the US?
Some did. The report notes that "some (USDA APHIS) staff members... argue that
because there is evidence that pigs, cats, mink, deer and a variety of
experimental animals may b e susceptible to trans. spongiform. encephalopathy,
the only prudent policy is to not feed products that contain these agents to ANY
SPECIES OF ANIMAL."
That's it. That's what we should have done then, and that's what we should
be doing now, but are not. Instead we are still exposing ourselves and US
livestock to a massive TSE experiment.
So, in 1991 the USDA and FDA rejected this advice by some anonymous
staffers, as they do today. This 1991 USDA PR report admitted that a more
cautious policy would be, quote, "to prohibit the feeding of sheep and
cattle-origin protein products to all ruminants, regardless of age. The
advantage of this option is that it minimized the risk of BSE. The disadvantage
is that the cost to the livestock and rendering industries would be
substantial."
In fact, absolutely nothing of substance was done until August of 1997,
when FDA announced its sham feed regulation, winner of the coveted Al Gore
hammer award, designed as a PR fig leaf so that FDA can say 'we are keeping
British mad cow disease out of our livestock."
Let me say that the only bright spot in this story of corporate and
government collusion, irresponsibility and censorship has been a few brave souls
like Richard Marsh, Richard Lacey in England, others who have bucked the tide
and spoken out, and those of you who are the families and loved ones of CJD
victims.
*** Through CJD Foundation and CJD Voice you have found each other, and you
will eventually force responsibility onto government and industry because if you
don't no one else will.
In conclusion, we need a massive commitment in the United States to address
TSE diseases. We need to start by admitting the shortcomings of our failed
federal feed policies and change them. We need to dedicate hundreds of millions
of research dollars, if necessary, to try to eradicate scrapie in sheep,
eradicate CWD in deer and in elk, and investigate, research, test and monitor
for TSEs in humans and animals. We need money for research to develop
treatments, because while this disease used to be rare disease, I suspect we are
about to see it emerge full blown in Britain with hundreds of thousands of
victims, and more and younger victims appearing in the United States, as a
result of our thirty year experiment in animal cannibalism.
The leadership for this will not come from politicians in bed with the
agribusiness industry. It will not come from researchers who while brilliant are
stuck back in pre-BSE days, still viewing this as a rare unusual disorder. It
will come from average citizens who husbands and mothers and children are dying
of this bizarre class of dementia diseases in increasing numbers, and who are
not afraid to demand that the right policies be implemented and funded. It is
upon your shoulders that leadership falls, and so far you are doing admirably."
snip...please see this and more here ;
Thursday, November 12, 2015
Evaluation of the protection of primates transfused with variant
Creutzfeldt-Jakob disease–infected blood products filtered with prion removal
devices: a 5-year update
Terry S. Singeltary Sr.
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