vCJD transfusion-associated Fourth Case UK

About Me

My Photo
Name:
Location: BACLIFF, Texas, United States

My mother was murdered by what I call corporate and political homicide i.e. FOR PROFIT! she died from a rare phenotype of CJD i.e. the Heidenhain Variant of Creutzfeldt Jakob Disease i.e. sporadic, simply meaning from unknown route and source. I have simply been trying to validate her death DOD 12/14/97 with the truth. There is a route, and there is a source. There are many here in the USA. WE must make CJD and all human TSE, of all age groups 'reportable' Nationally and Internationally, with a written CJD questionnaire asking real questions pertaining to route and source of this agent. Friendly fire has the potential to play a huge role in the continued transmission of this agent via the medical, dental, and surgical arena. We must not flounder any longer. ...TSS

Thursday, January 26, 2012

Prionemia and Leukocyte-Platelet-Associated Infectivity in Sheep Transmissible Spongiform Encephalopathy Models

Prionemia and Leukocyte-Platelet-Associated Infectivity in Sheep Transmissible Spongiform Encephalopathy Models



Caroline Lacrouxa, Didier Vilettea, Natalia Fernández-Borgesb, Claire Litaisea, Séverine Lugana, Nathalie Moreld, Fabien Corbièrea, Stéphanie Simond, Hugh Simmonse, Pierrette Costesa, Jean-Louis Weisbeckerf, Isabelle Lantierg, Frederic Lantierg, François Schelchera, Jacques Grassid, Joaquin Castillab,c and Olivier Andréolettia


+ Author Affiliations


aUMR INRA ENVT 1225, Interactions Hôtes Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France


bCIC bioGUNE, Parque Tecnológico de Bizkaia, Derio, Spain


cIKERBASQUE, Basque Foundation for Science, Bilbao, Spain


dCEA, Service de Pharmacologie et d'Immunoanalyse, IBiTec-S, DSV, CEA/Saclay, Gif sur Yvette, France


eVLA Weybridge, ASU, New Haw, Addlestone, Surrey, United Kingdom


fINRA Domaine de Langlade, Pompertuzat, France


gINRA IASP, Centre INRA de Tours, Nouzilly, France


ABSTRACT


The dynamics of the circulation and distribution of transmissible spongiform encephalopathy (TSE) agents in the blood of infected individuals remain largely unknown. This clearly limits the understanding of the role of blood in TSE pathogenesis and the development of a reliable TSE blood detection assay. Using two distinct sheep scrapie models and blood transfusion, this work demonstrates the occurrence of a very early and persistent prionemia. This ability to transmit disease by blood transfusion was correlated with the presence of infectivity in white blood cells (WBC) and peripheral blood mononucleated cells (PBMC) as detected by bioassay in mice overexpressing the ovine prion protein PrP (tg338 mice) and with the identification of abnormal PrP in WBC after using protein misfolding cyclic amplification (PMCA). Platelets and a large variety of leukocyte subpopulations also were shown to be infectious. The use of endpoint titration in tg338 mice indicated that the infectivity in WBC (per ml of blood) was 106.5-fold lower than that in 1 g of posterior brainstem sample. In both WBC and brainstem, infectivity displayed similar resistance to PK digestion. The data strongly support the concept that WBC are an accurate target for reliable TSE detection by PMCA. The presence of infectivity in short-life-span blood cellular elements raises the question of the origin of prionemia.


FOOTNOTES Received 11 October 2011. Accepted 18 November 2011. Address correspondence to O. Andreoletti, o.andreoletti@envt.fr.


Published ahead of print 7 December 2011


Copyright © 2012, American Society for Microbiology. All Rights Reserved.








Wednesday, August 24, 2011

All Clinically-Relevant Blood Components Transmit Prion Disease following a Single Blood Transfusion: A Sheep Model of vCJD
http://transmissiblespongiformencephalopathy.blogspot.com/2011/08/all-clinically-relevant-blood.html



Wednesday, August 24, 2011

There Is No Safe Dose of Prions
http://transmissiblespongiformencephalopathy.blogspot.com/2011/08/there-is-no-safe-dose-of-prions.html






Tuesday, September 14, 2010

Transmissible Spongiform Encephalopathies Advisory Committee; Notice of Meeting October 28 and 29, 2010 (COMMENT SUBMISSION)
http://tseac.blogspot.com/2010/09/transmissible-spongiform_14.html




Monday, February 7, 2011

FDA’s Currently-Recommended Policies to Reduce the Possible Risk of Transmission of CJD and vCJD by Blood and Blood Products 2011 ???
http://tseac.blogspot.com/2011/02/fdas-currently-recommended-policies-to.html




Thursday, December 29, 2011

Aerosols An underestimated vehicle for transmission of prion diseases?

PRION www.landesbioscience.com

please see more on Aerosols and TSE prion disease here ;
http://transmissiblespongiformencephalopathy.blogspot.com/2011/12/aerosols-underestimated-vehicle-for.html



Tuesday, January 17, 2012


American Red Cross Fined $9.6 Million for Blood-Safety Lapses AGAIN


http://transmissiblespongiformencephalopathy.blogspot.com/2012/01/american-red-cross-fined-96-million-for.html

 
 
 

TSS

Labels:

posted by Terry S. Singeltary Sr. | 11:13 AM | 0 Comments